Exam Frequency Analysis
Past paper frequency (2018 to 2024)
This topic accounts for approximately 12% of your exam marks.
stable
High
Stable12%
Food chains, energy transfer, and ecological definitions are regularly tested, often as short-answer questions.
Nitrogen is needed by every living thing to make proteins (and DNA, which contains nitrogen-rich bases). The atmosphere is 78% nitrogen gas (N₂), but this form is chemically unreactive and most organisms cannot use it directly. The nitrogen cycle shows how nitrogen is converted into usable forms and back again.
The four key processes
| Process | What it does | Carried out by |
|---|---|---|
| Nitrogen fixation | Converts N₂ from the air into ammonium (NH₄⁺) and then nitrate ions (NO₃⁻) that plants can use | Nitrogen-fixing bacteria in soil and in legume root nodules; also lightning; also industrial fertiliser production |
| Nitrification | Converts ammonia (from decomposition) into nitrites, then into nitrates | Nitrifying bacteria in the soil |
The full cycle
- N₂ gas in the atmosphere is converted to nitrate in the soil by nitrogen-fixing bacteria. These bacteria come in two main types:
- Free-living bacteria in the soil (e.g. Azotobacter)
- Mutualistic bacteria (e.g. Rhizobium) living in root nodules of leguminous plants (peas, beans, clover, lentils). These plants make excellent crops in rotation because they enrich the soil with nitrogen.
- Plants absorb nitrate from the soil through their roots by active transport.
- Plants use the nitrate to build amino acids and then proteins.
- Animals eat plants (or other animals), taking nitrogen-containing molecules into their own bodies and building their own proteins from them.
- Animals excrete nitrogenous waste (mostly urea) and eventually die.
- (bacteria and fungi) break down dead bodies and waste, releasing ammonia into the soil.
- convert ammonia → → , returning the nitrogen to a form plants can use.
Named bacteria you should know
| Group | Job | Example |
|---|---|---|
| Nitrogen-fixing bacteria | Fix N₂ gas from the air into ammonium/nitrate compounds | Rhizobium (in legume root nodules), Azotobacter (free-living) |
| Nitrifying bacteria | Convert ammonia into nitrites then nitrates | Nitrosomonas (ammonia → nitrite), Nitrobacter (nitrite → nitrate) |
| Denitrifying bacteria | Convert nitrates back to N₂ gas |
Why farmers manage the nitrogen cycle
Crops remove nitrogen from the soil with every harvest. If farmers do nothing, the soil eventually runs out of nitrogen and crop yields fall. Solutions include:
- Adding fertiliser that contains nitrate ions (industrially fixed nitrogen)
- Crop rotation with legumes, which restore nitrogen via their root-nodule bacteria
- Ploughing waterlogged fields to introduce oxygen and reduce denitrification (denitrifying bacteria need anaerobic conditions)
- Spreading manure, which decomposers convert to ammonia and then nitrate
Exam tip
Bacteria in the nitrogen cycle
Naming the process or bacteria for each step of the nitrogen cycle comes up, so you need to know: nitrogen fixation (nitrogen-fixing bacteria: N₂ → ammonium/nitrates), nitrification (nitrifying bacteria: ammonia → nitrite → nitrate), denitrification (denitrifying bacteria: nitrate → N₂), and decomposition (decomposers release ammonia). Don't muddle nitrification (builds nitrate) with denitrification (removes it).